S-STEM/TRAIN Scholar Jasmine Patricio

The Protocol that Josh had handed me was much more understandable than the first one that I was working on during the summer, the only problem with this was that the measurements were not listed on the protocol, so I had to solve for those myself, (with the help of Brian). 1. Measure 120 mL of DI water in 100 mL graduated cylinder. 2. Measure .3502 g of NaCl 3. Pour the .3502 g of NaCl into the 120 Ml of DI water 4. Pour the 120 ML of DI water + NaCl into 400 mL with 40 mL of the egg white 5. In a 50 mL measure 12 mL 15 mL of DI water place in Falcon Tube 6. Measure 10 mL of 100% Ethanol 7. Put it in the Falcon Tube 8. Grab a pipit and add add drops of Acetic Acid into teh 120 mL of DI water + .3502g NaCl+ 40 mL eggwhite, all into the 300 mL beaker. 9. Poured Acetic Acid until its PH was 4.06 What I have written down was mostly the measurements of everything since the protocol I was given didn't really have ant measurements of how much of everything I was suppose to use....

So this week I was continuing the protocol to making Lyzosome. Josh, Mat and Cory and I had decided that we needed a new needed to look at a different Protocol because the one that we had done during the summer had a lot of difficult stuff to it. And because of my poor note taking during the summer we were not able to duplicate what i had done over the summer. Also because during the summer the Protocol that we had, we mostly took educated guesses on what we were adding and contributing to the experiment.  Josh was able to find a new Protocol for me that he had found : http://www.epibio.com/ applications/nucleic-acid- purification-extraction-kits/ buccal-swabs-and-dna- extraction/quickextract- bacterial-dna-extraction-kit http://omegabiotek.com/store/ product/bacterial-dna-kit/ And that made it much simpler for me to follow. The only problem with that Protocol was that it didn't have that much any measurements for the chemicals that we were adding on. So we had to com...

Hello, My name is Jasmine I plan on attending a community college for two years and transferring to the University of Arizona Downtown or Arizona State University where I will be working to earn my bachelor's degree in biology and continue my pursuit to being an ophthalmologist.Though I like to think that I have my future set up, I have a tendency of changing my career choice multiple of times. One thing for sure that I know is that I want to pursue my goal, in the medical field. This summer 2016 I was given the opportunity to be part of program; “STEM Internship Program” which requires every intern to put in at least 9+ hours a week to complete a total of 60 hours in one month. During my internship I was given a bacteria which had an unknown name, and through out my 60+ hours of being at Phoenix college my objective was to find out the name of our bacteria through multiple test. Then try to extract DNA from the bacteria using PCR Protocol. We also created Lysozyme from extracti...

What I think my project will be is the continuation of purifying the Lysoszome. During the summer we didn’t have enough Lysoszome so we had to create more, the way we did that was extracting Eggwhite from a chicken egg and creating a protocol that was able to clean the egg white. In today’s meeting what we did was go over the Program and the requirements that we all have to fulfill in order to receive the full scholarship I thought the meeting was very helpful especially seeing that I was not the only one who was new to the S-Stem program. Also helped seeing some people who have done the program for over a period of time so that they can maybe help me find what I am truly interested in. What I get to get from working in the lab is get more experience in research labs and get more use to doing experiments. I also hope that I can maybe get into outside internships like maybe labs or even hospitals. The picture above is PCR Extraction of Lysozme from Chicken Eggwhite.