Blog Post Week 5

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My next step after purifying the lysozyme is to run it in the PCR. The only problem that I had is that we don’t have Master mix. I can’t run the experiment without Master mix without it because that is needed for me to run it in the PCR machine. PCR stands for Polymerase Chain Reaction, which is often used in biological and chemical labs. A thermal cycler, or PCR machine, has the ability to produce DNA copies of a specific segment that can range from thousands to millions in numbers. The basic function of this machine is copying the sections of DNA and this is performed through a heating cycle. This is performed when the temperature rises to 95 degree Celsius which in turn melts the DNA strands. This melting of DNA strands causes the backbones of sugar phosphate to split apart. Then as the temperature lowers, the primers bind them 3 inch end of each sequence of target. Primers are able to perform this task as the DNA polymerase taq and free nucleotides aid it in the process. This process goes on so that there are two strands of double partially stranded molecules of DNA at the end of first cycle. The same process continues to be repeated again and again causing thousands of copies of the particular target sequence.

Lysozyme extract → PCR you’re primer
  • 2 UL forward primer
  • 2 UL reverse primer
  • 10 UL 2x PCR Master Mix
  • 4 UL molecular mix
  • 2 UL of Lysozyme

References:
"What Is a PCR Machine?" What Is a PCR Machine? Amazon Services LLC Associates Program, n.d. Web. 02 Nov. 2016. 

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