Blog Post Week 6

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Nano Drop Graph
  1. Open the app
  2. Click “Nucleic Acid”
  3. Open arm
  4. Add 2 ul of TE Buffer
  5. Close arm
  6. Click “Blank”
  7. Open arm
  8. Add 2 ul of bacteria on top of the TE Buffer
  9. Close arm
  10. Name it
  11. Click measure
  12. Clean off
  13. Repeat for as many samples you have

A260/280 ratio The A260/280 ratio is generally used to determine protein contamination of a nucleic acid sample. The aromatic proteins have a strong UV absorbance at 280 nm. For pure RNA and DNA, A260/280 ratios should be somewhere around 2.1 and 1.8, respectively. A lower ratio indicates the sample is protein contaminated. The presence of protein contamination may have an effect on downstream applications that use the nucleic acid samples.

Black cap 1 & 2 = No removal of the pellet, so that mean in the two little ones they each contained DI H2O + S.M. + Quick Extract + EWL (Egg White Lysozyme)
No cap 1 & 2 = Removal of pellet, the two little tubes contain
Quick Extract + EWL (Egg White- Lysozyme)

Black cap #1 which was 1.8 had the most purity.

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