Blog Post Week 8

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Nanodrop (Micrococcus Luteus)
Black Cap #1,2 = DI-H20 + M.L.+ Quick Extract+ E.W.L
Clear Cap #1,2 = Quick Extract + E.W.L
Today, I performed a nanodrop on my new bacteria Micrococcus Luteus. My focus was on the nucleic acid. Nucleic acids are the main information-carrying molecules of the cell, and, by directing the process of protein synthesis, they determine the inherited characteristics of every living thing (n.p). The goal was to get around 200-280. Seeing that the two BlackCap are the closest to the numbers indicated means that there was more possible DNA in the Bacteria than ClearCap #1,2.

A 260/280 ratio of ~1.8 is generally accepted as “pure” for DNA;
A ratio of ~2.0 is generally accepted as “pure” for RNA.

Reference:

"nucleic acid". Encyclopædia Britannica. Encyclopædia Britannica Online.
Encyclopædia Britannica Inc., 2016. Web. 15 Nov. 2016
<https://www.britannica.com/science/nucleic-acid>.

Desjardins, Philippe, and Deborah Conklin. "NanoDrop Microvolume Quantitation of Nucleic Acids." Journal of Visualized Experiments JoVE -1 (2010): n. pag. Interpretation of Nucleic Acid 260/280 Ratios. Thermo Scientific. Web. 15 Nov. 2016. <http://www.mgp.cz/files/nanodrop/manualy/pomer_cistoty.pdf>.

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