Blog Post Week 3 : Developing a Protocol

The Protocol that Josh had handed me was much more understandable than the first one that I was working on during the summer, the only problem with this was that the measurements were not listed on the protocol, so I had to solve for those myself, (with the help of Brian).

1. Measure 120 mL of DI water in 100 mL graduated cylinder.
2. Measure .3502 g of NaCl
3. Pour the .3502 g of NaCl into the 120 Ml of DI water
4. Pour the 120 ML of DI water + NaCl into 400 mL with 40 mL of the egg white
5. In a 50 mL measure 12 mL 15 mL of DI water place in Falcon Tube
6. Measure 10 mL of 100% Ethanol
7. Put it in the Falcon Tube
8. Grab a pipit and add add drops of Acetic Acid into teh 120 mL of DI water + .3502g NaCl+ 40 mL eggwhite, all into the 300 mL beaker.
9. Poured Acetic Acid until its PH was 4.06

What I have written down was mostly the measurements of everything since the protocol I was given didn't really have ant measurements of how much of everything I was suppose to use.

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